Apperance:white to off-white, amorphous powder of low density
Solubility: Soluble in water at a concentration of ≥6 mg/ml to give a clear, colorless solution
Identity: Monoisotopic Mass: 1636.7 ±1 m.u.
(by Mass Special Analysis)
Identity
(by Mass Special Analysis)
Asx 0.95 to 1.05
Thr 1.4 to 201
Ser 0.7 to 1.05
Gly 0.95 to 1.05
Ala 0.95 to 1.05
Cys 1.4 to 2.1*
Phe 2.85 to 3.15
Lys 1.9 to 2.1
Trp 0.3 to 1.1
*Hydrolyze without phenol to recover Cysteine
Identity:
Coelutes with reference standard
System I (0.1% TFA/Acetonitrile)
Gradient=20-45% in 25 minutes
System II (0.1M TEAP, pH 2.3/Acetonitrile
Gradient=20-45% in 25 minutes
Identity
(By TLC)
RF value of sample correspondes to reference standard
Peptide purity: ≥98%
By Analytical HPLC
Related substance ≤2% total, no single purity > 1%
Water (karl Fisher) ≤ 8%
Specific Rotation -37o to -47o [α]D~20 c=0.5, 1% acetic acid)