Apperance:white to off-white, amorphous powder of low density
Solubility: Soluble in water and 1% acetic acid at a concentration of ≥1 mg/ml to give a clear, colorless solution
Identity: Monoisotopic Mass: 1018.4 ±1 m.u.
(by Mass Special Analysis)
Identity
(by Mass Special Analysis)
Thr 0.85 to 1.15
Cys 1.3 to 2.1*
Phe 1.7 to 2.3
Lys 1.3 to 2.1**
Trp Present
*Hydrolyze without phenol to recover Cys
**Thr-ol co-elustes with Lys
Identity:
Coelutes with reference standard
System I (0.1% TFA/Acetonitrile)
Gradient=15-40% in 25 minutes
System II (0.1M teap, pH 2.3/Acetonitrile
Gradient=10-35% in 25 minutes
Peptide purity: ≥98%
(By Analytical HPLC)
Related substance ≤2% total
Water (karl Fisher) ≤ 10%
Specific Rotation -17o to -21o [α]D~20 c=0.5, 1% acetic acid)
pH: 5-7